(Fireflysci.com: Farmingdale, NY) -- For several decades, spectrophotometry has been used in microbiology, clinical chemistry, the pharmaceutical industry, and other research applications.

Periodic verification of the performance of spectrophotometers is required by ISO 9001, good laboratory practice (GLP), good manufacturing practice (GMP), standard operating procedures (SOP) in the pharmaceutical industry, EP, DAB, USP (pharmacopeias), and other applicable standards and regulations.

Periodic verification of performance of spectrophotometers typically includes testing the resolution, wavelength accuracy, photometric accuracy, and stray-light affect. Traditionally, the calibration of spectrophotometers has been performed with a combination of liquid filters and solid-state filters. The solid-state filters only allowed the checking of photometric accuracy and wavelength accuracy usually within the 450 nm – 630 nm range. The liquid filters allow checking the photometric accuracy, wavelength accuracy, stray-light affect, and photometric resolution usually within the 200 nm – 660 nm range.

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